Skills

 

MICHAEL V. KEEBLER

Ph.D.

PROFESSIONAL SKILLS

SCIENTIFIC

Molecular Biology

  • CRISPR engineering of mammalian cell-lines (monomeric and dimeric, HDR and NHEJ strategies)
  • Conventional cloning, TOPO cloning, Gibson assembly,
  • Point mutagenesis
  • Genotyping
  • Recombineering (PiggyBac)
  • In silico tools: Lasergene, UCSC, Ensembl, NCBI, BioEdit, ExPASy, Clustal, Primer3, ZiFit CRISPR (Zhang, MIT), WTSI Genome Editing, E-CRISPR, GPP (Broad)
  • qPCR

 

Cell Culture

  • Mouse embryonic stem cells
    • 129 strain, feeder-free, N2B27 medium.
  • Human iPS stem cells
    • SNL/STO feeders, EPSC, CDM and KSR + FGF mediums.
  • Immortalized, adherent cell lines
    • HEK293, Hela, U2OS, MLO-Y4, S2R+, MDA-MB-231, HCC 1569, SUM159
  • FACS
    • Influx, Flo Legacy, MoFlo and BioRad S3 sorters
  • Single-cell expansion
    • Limiting dilution, selection/picking or sorting

 

Biochemistry

  • Large scale production, purification and concentration of soluble protein
  • Column chromatography
    • Affinity, ion-exchange and size-exclusion
  • Protein assaying
    • Bradford, Western, silver/coomassi stain, Luciferase activity, denistometry
  • 3D structural analysis (PyMol)

 

High-resolution Imaging

  • Total Internal Reflection Fluorescence (TIRF)
  • High-speed Spinning Disk Confocal
  • Prolonged live-cell imaging with repeated drug stimulation
  • Single-molecule measurement of ligand/protein interactions
  • Image analysis (ImageJ, Amira, R^cell, Metamorph)

 

Cell Physiology

  • High-throughput Ca2+ measurements
    • 96-well FLEX station, single wave-length indicator
  • Single-cell Ca2+ responses at the population level
    • Mounted slides, single or dual wave-length indicators.
  • High-resolution (TIRF) measurements of elementary Ca2+ events (puffs)

 

Animal Handling

  • Sprague Dawley rat
    • Survival surgery (neural)
  • Xenopus laevis
    • Microinjection in oocytes

 

Radioligand Binding

  • Competition binding (3H-IP3)

 

Programming & Computational Biology

  • IDL (3 large-scale algorithms written)
  • R (1 large-scale algorithms written)
  • Python, MATlab, Java, Octave (familiarity/proficiency)
  • Organization, cleaning and formatting of large data sets
  • NGS analyses, including Rsamtools and GATK
  • Familiarity with Machine Learning algorithms and theory (Andrew Ng’s course, Coursera)

 

NON-SCIENTIFIC

Leadership and Management

  • St. John's College Samual Butler Room (SBR)

Academic Officer - 2013-2014

Social Dining Officer - 2012-2013

  • Cambridge University Real Ale Society

President - 2012-2014 (re-elected)

  • University of Minnesota Vo Lam Kung Fu

President - 2007-2008

Treasurer - 2006-2007

  • KUOM Radio K

Chief Student Engineer - 2007-2008

Head of Engineering Department - 2007-2008

 

Communication

  • Teaching
    • University of Cambridge

Science Festival Demonstrator - 2015

YoungPharmas Lecturer/Demonstrator - 2011-2015

Pharmacology Demonstrator - 2011-2015

  • STEMNET

STEMNET Ambassador - 2014

  • REACH Cambridge

Microscopy Lecturer - 2013-2014

  • Wellcome Trust & Goldsmiths' Company

Advance Genetics Course Demonstrator and Lecturer - 2012-2014

  • University of Minnesota

Teaching Assistant - Cell Biology 4004 - 2009

  • Society for Neuroscience

Brain Awareness Week Volunteer - 2009

  • Presentations
    • International

IFOM (Italy) - 2015

Wellcome Trust Sanger Institute (UK) - 2015

Trinity College Dublin (Ireland) - 2014

  • University of Cambridge

Department of Pharmacology - 2011-2014

St. John's College - 2014

  • University of Minnesota

Department of Neuroscience - 2008

College of Biological Sciences - 2008

  • Strategy Meetings
    • Scientific Industry

Cancer Research Technologies (bi-monthly) - 2016

  • Collaborators

Dr. Walid Khaled (Cambridge) - 2015-2016

Dr. Jennifer Nichols (Cambridge) - 2014-2015

Dr. Andrew Riley (Bath) - 2010-2015

Dr. John Burk (LMB) - 2013-2014

Dr. Mark Wallace (Oxford) - 2011

Dr. Sandip Patel (UCL) - 2009-2010

  • Writing
    • Publications

PhD thesis

Undergraduate honors thesis

2x first authored publications (in preparation)

6x co-authored publications

1x review

1x book chapter

 

Teamwork

  • Science

Collaborations (see above)

  • Sports

Cambridge University Amature Boxing Club (CUABC) - Blues Team, 2015-2016

  • Societies

St. John's College Samual Butler Room (SBR)

Cambridge University Real Ale Society

University of Minnesota Vo Lam Kung Fu

KUOM Radio K

 

 

Additional Traits

Project Management

  • Independently planned and executed:

2 projects during my Postdoctoral Research Fellowship

5 projects during my PhD

 

Problem solving

  • Trouble shot the development and realization of at least 8 new, large-scale techniques and protocols.

Networking

  • Initiated 2 inter-lab collaborations by establishing contact and exchanging ideas.
  • Organized a departmental seminar on cell signaling to foster discussion between labs.

 

Initiative

  • Revolutionized my department by introducing stem cell and CRISPR/Cas9 technologies.
  • Took initiative to learn programming without formal training in order to solve a scientific problem

 

 

CONTACT DETAILS

 

michael.v.keebler@gmail.com

 

Tel: +44 (0)1223 834244

© 2016 Michael V. Keebler. All rights reserved.