Experience

 

MICHAEL V. KEEBLER

Ph.D.

EDUCATION

University of Cambridge

Doctor of Philosophy (Ph.D.)

Thesis title: High-resolution analyses of inositol 1,4,5-trisphosphate receptor behavior

2011 - 2015

2004 - 2009

University of Minnesota

Bachelors of Science (B.S.)

Major: Neuroscience

Major: Genetics, Cell Biology & Development (GCD)

 

RESEARCH EXPERIENCE

Postdoctoral Fellow

Wellcome Trust Sanger Institute - 2015 - 2016

  • Led a translational collaboration between the Wellcome Trust Sanger Institute, Cancer Research Technology and the Department of Pharmacology (University of Cambridge). The industrial nature of this position required meeting tight deadlines and frequently presenting results to members of the funding body.
  • Demonstrated accurate gene editing using CRISPR/Cas9 on cells with limited homology-directed repair (the machinery required for conventional gene-editing).
  • Developed protocols for the efficient knock-in of cancer genomes, which are difficult to target.

PhD Student

University of Cambridge - 2011 - 2015

  • Created two large-scale algorithms (written in IDL) for the automated detection and analysis of inositol-1,4,5-trisphosphate (IP3)-evoked Ca2+ release events and sites in mammalian cells.
  • Discovered a new population of ‘low-frequency’ Ca2+ release sites in mammalian cells.
  • Provided new evidence to support the argument that globally delivered IP3 evokes Ca2+ release from many of the same sites as agonist delivered IP3.
  • Dramatically improved the protocols in the lab for prolonged stimulation with agonist in mammalian cells.
  • Characterized IP3-evoked Ca2+ release events/sites in HEK293 cells for the first time.
  • Synthesized N-terminal fragments of the IP3 receptor (IP3R) with a high purity/concentration for structural-function analysis by deuterium exchange mass spectrometry.
  • Measured the interaction between IP3 receptors (IP3R) and fluorescent-IP3 at the single-molecule level using total internal reflection fluorescence (TIRF) microscopy.

 

Research Assistant

University of Cambridge - 2010 - 2011

  • Pioneered single-molecule fluorescence microscopy in the lab by creating an assay for measuring the interaction between an individual molecule of the IP3R and an individual molecule of fluorescent-IP3.

Research Assistant

University of Minnesota - 2006 - 2009

  • Discovered that humans can perceive pitch beyond the traditionally accepted limit (6kHz).
  • Established that humans do not utilize the temporal fine structure of a harmonic complex wave to process unresolved harmonics.
  • Discovered that pitch intervals do not offer any perceptual advantage compared to other acoustical dimensions (e.g. loudness and spectral brightness).
  • Demonstrated that tracking the pitch of one tone (in the presence of a competing tone), requires salient peaks in the excitation pattern of the basilar membrane after summing the two tones together.

 

Research Specialist

University of Minnesota - 2009 - 2010

  • Characterized the sub-cellular localization and morphology of two-pore channel (TPC) isoforms overexpressed in the oocytes of Xenopus laevis.
  • Identified a pigmentation phenotype associated with TPC2 expression, which helped identify the role of the TPC complex in endolysosomal trafficking.

Research Assistant

University of Minnesota - 2008 - 2009

  • Created a three-dimensional reconstruction of the mouse inner ear from images acquired via thin-sheet laser imaging microscopy (TSLIM).

CONTACT DETAILS

 

michael.v.keebler@gmail.com

 

Tel: +44 (0)1223 834244

© 2016 Michael V. Keebler. All rights reserved.